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KMID : 0384119900100020403
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Korean Journal of Clinical Pathology
1990 Volume.10 No. 2 p.403 ~ p.410
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Detection of Serum Platelet Antibodies Using Microplate Platelet Suspension Immunofluorescence Test
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W. I. Oh
M. H. Park/K. S. Han
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Abstract
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We set up improved platelet suspension immunofluorescent test(PSIFT) using U` microplate and paraformaldehyde(PFA)- fixed and stored platelets. PFA- fixed stored platelets could be used for 4-5 weeks without loss of antigenicity. Using PFA- fixed stored platelets, time for collection and preparation of platelets could be saved, shortening the assay time by 2 hours. In addition, stored platelets showed less aggregating tendency making washing procedure easier compared to freshly prepared PFA- fixed platelets. Using microplate, the test procedure could be easily performed for a larger number of samples, compared to tube method. Using this Microplate- PSIFT method, the positive rate of serum platelet antibody was examined in 228 patients with various clinical disorders. In patients with autoimmune diseases, serum platelet antibodies were positive in 54% (28/52) of patients with ITP, 74% (17/23) of patients with SLE and 64% (7/11) of patients with other autoimmune diseases. Serum platelet antibodies were detected in 41% (14/34) of patients with aplastic anemia among which platelet antibodies were positive in 72% of previously diagnosed patients in contrast to 6% of freshly diagnosed patients. Of 108 cases with various diseases other than autoimmune diseases, 30% were positive in platelet antibody test, and the majority of the positive cases were considered to be transfusion related. Serum platelet antibodies were positive in 77% of patients showing refractoriness to platelet transfusion and in 68% of patients with multiple platelet transfusion history. The microplate- PSIFT method used in this study has several advantages compared to conventional PSIFT method using freshly prepared platelets and tube. And the sensitivity of the test is satisfactory in various clinical disorders including autoimmune thrombocytopenias.
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KEYWORD
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